AFFI looks at current detection methods for public health, regulatory policy

September is Meals Security Schooling month and, in recognition, the American Frozen Food Institute (AFFI) and the International Fresh Produce Association (IFPA) will host a digital Food Safety Forum, the place they are going to convene world analysis consultants, business professionals, and meals security coverage leaders to debate rising points associated to non-cultivable foodborne pathogens. 

Enteric viruses similar to hepatitis A, norovirus and protozoan parasites like Cyclospora and Cryptosporidium are foodborne pathogens related to several types of meals, and which have been implicated in outbreaks. Considerations about these pathogens have led to requires extra routine product testing and surveillance throughout the meals provide chain; nevertheless, detection of those pathogens has a number of limitations.

Audio system on the Meals Security Discussion board will talk about technical challenges and regulatory questions related to detection of those pathogens and interpretation of outcomes.

Following are excerpts from interviews with IFPA’s Jennifer McEntire, Chief Meals Security & Regulatory Officer, and AFFI’s Sanjay Gummalla, Senior Vice President of Scientific, discussing the importance of the discussion board and the scientific discussions from the angle of meals security and public well being.

Query: Why is the Meals Security Discussion board addressing non-cultivable foodborne pathogens now and why are AFFI and IFPA encouraging participation from business, authorities and academia?

McEntire (IFPA): Whereas concern has grown over the prevalence of those pathogens in meals, they current distinct detection points not seen with bacterial pathogens. This discussion board will delineate variations between bacterial and non-cultivable pathogens similar to hepatitis A, norovirus and Cyclospora. For example, they can’t be propagated by way of pre-enrichment, selective enrichment, or selectively plated, that are gold requirements utilized in identification of bacterial pathogens similar to Salmonella or Listeria. An consciousness of this matter is related to all meals security professionals. 

Gummalla (AFFI): In contrast to bacterial pathogens that may be grown to excessive numbers in a lab, enteric viruses should be remoted from the meals or atmosphere by focus and purification, adopted by nucleic acid extraction, earlier than a detection methodology similar to PCR may be utilized. The discussion board will assist attendees higher perceive these steps and the implication of optimistic findings, and the regulatory and public well being concerns. All stakeholders ought to have an improved understanding of this matter as requires meals product testing enhance.  

​Query: Do these pathogens develop in meals? If we can not tradition these pathogens and they don’t develop in meals, how will we confirm contamination?

Gummalla (AFFI):  No, they don’t develop in meals and that may be a good factor. However since these pathogens can’t be grown, the query we have to ask ourselves is, “is with the ability to detect a nucleic acid fragment synonymous with contamination?” Whereas a suspected bacterial pathogen similar to E. coli or Listeria monocytogenes may be cultivated in a laboratory to verify presence and viability, there isn’t a such risk with non-cultivable pathogens. As an alternative, we use PCR-based strategies, like testing for the detection of SARS-CoV-2 in medical settings.

McEntire (IFPA): In FDA’s BAM detection protocol for Cyclospora, the company does define sure PCR thresholds, however there stay questions concerning the reliability of PCR testing in meals the place these pathogens are each heterogeneously distributed and should happen solely in low numbers. This case results in a posh and ambiguous regulatory interpretation of “what” constitutes “an indicator of contamination” and whether or not a nucleic acid discovering determines contamination or adulteration.  ​ 

Query: How is pattern positivity at present decided?

Gummalla (AFFI): Whereas a PCR check sign could also be optimistic, proof for meals contamination stays unclear. Moreover, confirmatory approaches can assist the unique PCR-based optimistic discovering, however none of those strategies have been adequately vetted, revealed or routinely utilized by the technical neighborhood.

McEntire (IFPA):  For Cyclospora particularly, the organism seems to have a posh life cycle, and is just infectious throughout one part of its life. So even when there’s a PCR optimistic, you may’t inform which model of the organism was there, even whether it is viable.

Query: What different elements can inform danger?

McEntire (IFPA): Given the restrictions of testing and the uncertainty with what a optimistic check for the presence of nucleic acid could imply, there are a number of different elements that must be assessed through the assessment of a doubtlessly optimistic PCR check end result. This might embrace a assessment of the presence of employee sicknesses on the farm or facility, a assessment of website sanitation and the usage of Good Manufacturing Practices (GMP) or Good Agricultural Practices (GAP). You will need to perceive the totality of public well being danger to tell meals security choices. 

​​Query: What sources can be found to help these communities decreasing their danger for these organisms?

Gummalla (AFFI): AFFI just lately launched an Enteric Virus Management Specialist certification program in partnership with the Worldwide Meals Safety Coaching Institute. This consists of programs that tackle finest practices in employee well being and hygiene, managed use of water, waste administration, and sanitation of kit and instruments. The course is predicated on AFFI’s Enteric Virus Control Program, a free useful resource accessible to all produce growers and processors.

McEntire: IFPA and AFFI are working intently with FDA in assist of business prevention methods for particular product- hazard pairs, together with berries and hepatitis A, and we’ll share any new data or insights gained. For Cyclospora, we have now a technical bulletin free for business use. However in truth, we have to higher perceive this organism and routes of contamination. We don’t want the business to waste sources implementing applications that gained’t have an effect.

Query: Why ought to meals security consultants be desirous about studying extra about non-cultivable foodborne pathogens?

Gummalla (AFFI): We’re on the crossroads of setting vital precedents in our approaches to figuring out “indicators of contamination,” “adulteration,” and ascribing “public well being danger” related to non-cultivable pathogens. It will be important for all stakeholders within the meals security neighborhood to grasp limitations in strategies, how we assess contamination, and the regulatory enforcement and public well being impression. We’re excited to host the Meals Security Discussion board and drive scientific consciousness, debate and understanding of this difficult matter. We welcome meals business professionals to hitch us nearly on September 21, 2022, at 11 a.m. Japanese.  

​This data was contributed by AFFI. For extra data see  

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